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| 产品名称： | pCNV220 |
|---|---|
| 商品货号： | TS143258 |
| Designations： | pCNV220 |
| GenBank Number： | M25270 |
| Species： | Cucumber necrosis virus (CNV) |
| Depositors： | DM Rochon |
| Applications： | contains sequence genome, 90%+ complete |
| Vector： | Construct size (kb): 7.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.699999809265137 Gene product: genome, 90%+ complete Target Gene: genome, 90%+ complete |
| Insert Size (kb)： | 4.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--4.6, 3.0; HindIII--3.5, 1.4, 1.3, 1.2; BglI--2.7, 1.7, 1.5 (doublet). Insert contains the following restriction sites (approximate kb from the 5 end): BglI--2.66, 4.29; BglII--3.36; EcoRI--2.14; EcoRV--1.07, 1.49, 1.57, 3.19, 4.03; HindIII--0.62, 1.80, 3.22; KpnI--3.89; NdeI--0.64, 1.58, 3.52, 3.63; PvuII--2.49, 3.33. Contains entire coding sequence plus 20 nucleotides of 5 noncoding sequence. Virus was isolated from cucumber, Cucumis sativus (cultivar strain 8). |
| Classification： | Tombusvirus |
| References： | Rochon DM, Tremaine JH. Complete nucleotide sequence of the cucumber necrosis virus genome. Virology 169: 251-259, 1989. PubMed: 2705296 DAnn M Rochon, personal communication |