Construct size (kb): 4.900000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD1*
Gene name: CD1 antigen, R3
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.65
Cross references: DNA Seq. Acc.: T08190; EST: EST06081; Primer: M13-21; THC
id: THC118159
DNA Seq. Acc.: T08191; EST: EST06082; Primer: M13 Reverse;
THC id: THC119203
DNA Seq. Acc.: X14974; EST: EST06082
Nucleotides 1-259, 260-361 of the insert correspond to
nucleotides 5559-5817, 5817-5918 of X14974 with 99% identity.

Insert lengths(kb): 1.649999976158142

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: CD1 antigen R3 CD1*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 0.87; HindIII/NotI--3.4, 1.65.

Verified to contain EST06081 by single-pass sequencing. This EST does not at present match a nucleotide sequence record.

Based on the size of the insert and the alignment with GenBank X14974 (genomic, last exon 4483-5598, end of CDS 4854), this clone appears to be derived from the 3 untranslated region and extends beyond previously reported sequence.