微信小陈
微信小章
产品简介
购买须知
| 产品名称： | SM38 |
|---|---|
| 商品货号： | TS143283 |
| Designations： | SM38 |
| GenBank Number： | X14420 |
| Species： | Homo sapiens, human |
| Depositors： | H Kuivaniemi |
| Vector： | Construct size (kb): 7.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: COL3A1 Genomic copy number: unique Gene name: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) Contains complete coding sequence?: N Chromosome: 2; Localization: 2 q31-q32.3 Tissue: skin fibroblasts, neonatal (cell line JIMM-69) Type of DNA: cDNA Insert 5 end: Modification: EcoRI linker Insert 3 end: EcoRI Insert size (kb): 4.278 Cross references: DNA Seq. Acc.: X14420 Nucleotides 1-4278 of the insert correspond to nucleotides 25-4303 of X14420. Insert lengths(kb): 4.277999877929688 Tissue: skin fibroblasts, neonatal (cell line JIMM-69) Gene product: collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) COL3A1 |
| Insert Size (kb)： | 4.278 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.4(doublet), 0.9; BglI-- 2.5, 1.4(doublet), 0.9(doublet), 0.52; EcoRI--4.6, 2.9; NcoI--4.7, 3.5; XhoI--3.9, 2.5, 1.1. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.25, 1.11; BglI--0.61, 1.94, 2.39, 3.28; EcoRI--4.27; NcoI--0.97, 4.07; PvuII--0.60, 0.84, 2.39, 3.09, 3.25, 4.13; SacI--0.96, 1.29, 2.08, 2.90; XhoI--1.05, 3.57. The insert contains 78 nt 5 untranslated sequence, and 4200 nt of the coding region. The insert ends at a naturally-occuring EcoRI site at nt 4303 of the sequence record. |
| References： | Tromp G, et al. A single base mutation that substitutes serine for glycine 790 of the alpha1(III) chain of type III procollagen exposes an arginine and causes Ehlers-Danlos syndrome IV. J. Biol. Chem. 264: 1349-1352, 1989. PubMed: 2492273 |