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| 产品名称： | Human adenovirus D |
|---|---|
| 商品货号： | TS143317 |
| Classification： | Adenoviridae, Mastadenovirus |
| Agent： | Human adenovirus D |
| Strain： | A.A. |
| Common Name： | Human adenovirus 13 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Recognized Reference Strain: Pereira HG, et al.A short description of adenovirus group. Virology 20: 613, 1963. Agglutinates both rat and rhesus RBC but usually the former to a higher titer than the latter. Contains soluble group-specific complement fixing antigen. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | KB cells (ATCC® CCL-17™) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 60-90% confluent. Remove medium and inoculate with a small volume (e.g. 1mL per 25 cm squared) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 1-2 hours at 37°C in a 5% CO2 atmosphere. End adsorbtion by adding VGM.
Incubation: Incubate infected culture for 1-6 days at 37°C in a 5% CO2 humidified atmosphere until CPE is well advanced through 90% of culture. |
| Name of Depositor： | RJ Huebner, NIH |
| Chain of Custody： | Deposited as Adenovirus type 13 |
| Source： | Anal swab from healthy child, Washington, DC, 1955 |
| References： | Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Rowe WP, et al. Serotype composition of the adenovirus group. Proc. Soc. Exp. Biol. Med. 97: 465-470, 1958. PubMed: 13518306 |