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微信小章| 产品名称： | Human herpesvirus 5 |
|---|---|
| 商品货号： | TS143324 |
| Classification： | Herpesviridae, Cytomegalovirus |
| Deposited As： | Human Cytomegalovirus, recombinant Towne strain |
| Agent： | Human herpesvirus 5 |
| Strain： | RC256 |
| Common Name： | Human cytomegalovirus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | Recombinant derivative of the attenuated Towne strain, which carries and expresses the E. coli lacZ gene. This material is cited in a U.S. and/or other patent and may not be used to infringe the patent claims. Recombinant virus produces focal CPE, which appears as blue plaques when overlayed with X-Gal (150 µg/mL in serum supplemented D-MEM with 0.5% agarose). |
| Effect on Host： | CPE, cell enlargement, rounding and degeneration |
| Recommended Host： | MRC-5 cells (ATCC® CCL-171™) |
| Growth Conditions： | Recommendations for Infection: Infect host cells in a T-25 flask or equivalent culture vessel, at 80-90% confluence.xa0xa0 Incubate for 2 to 9 days at 37°C in a humidified 5% CO2 atmosphere.xa0 For passage, co-cultivate infected cells with fresh cells.xa0 xa0For preservation, harvest infected cells by trypsinization, resuspend in culture fluids supplemented with DMSO to 8%, and freeze. |
| Name of Depositor： | E Mocarski |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | In vitro recombination of viral DNA and and a plasmid carrying the E. coli lacZ gene driven by a HCMV b promoter |
| References： | Spaete RR, Mocarski ES. Insertion and deletion mutagenesis of the human cytomegalovirus genome. Proc. Natl. Acad. Sci. USA 84: 7213-7217, 1987. PubMed: 2823255 |