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| 产品名称： | Influenza A virus (H1N1) |
|---|---|
| 商品货号： | TS143328 |
| Classification： | Orthomyxoviridae, Influenzavirus A |
| Agent： | Influenza A virus (H1N1) |
| Strain： | A/Virginia/ATCC3/2009 |
| Applications： | Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This item was isolated in tissue culture. |
| Product Format： | frozen |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin. |
| Effect on Host： | CPE, rounded refractile cells with cell degeneration |
| Recommended Host： | MDCKxa0(ATCC® CCL-34™) |
| Growth Conditions： | Temperature: 35°C Recommendations for Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI 0.1. Adsorb 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Grow in serum free media that contains 2.0μg/ml TPCK-treated trypsin. Incubation: 2-5 days |
| Name of Depositor： | ATCC |
| Special Collection： | ATCC |
| Source： | Nasopharyngeal specimen from a patient positive for Flu A in Virginia, 2009. |