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微信小章| 产品名称： | phi6-3 |
|---|---|
| 商品货号： | TS143462 |
| Designations： | phi6-3 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Applications： | Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information. |
| Vector： | Construct size (kb): 44.0 |
| Insert： | DNA: genomic Insert lengths(kb): 14.5 Gene product: DNA Segment, single copy D11S85 Alleles: A1, B2, A2, B1 |
| Insert Size (kb)： | 14.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 12.5, 2.0; SalI--19.5, 14.5, 10.0. phi6-3 contains repeated sequences and must be prehybridized with total human DNA before it will detect single copy bands. Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C. MspI detects 2 diallelic RFLPs. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information. |
| References： | Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085 Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469 Maslen CL, et al. Seven polymorphic loci mapping to human chromosomal region 11q22-qter. Genomics 2: 66-75, 1988. PubMed: 2898428 Michael Litt, personal communication |