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| 产品名称： | pT109luc Plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS143495 |
| Designations： | pT109luc Plasmid in Escherichia coli |
| Depositors： | SK Nordeen |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.09
Vector: pT109luc (plasmid)
Promoters: Promoter HSV TK
Construction: pXP2, herpes simplex virus TK promoter
Marker(s):ampR
Construct size (kb): 6.09
Features: marker(s): ampR promoter: HSV TK replicon: pMB1 terminator: none enhancer: none |
| Applications： | xa0expression vector
xa0shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6. There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts. This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California. One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells. This contains bp -109 to +52 of the herpes simplex tk promoter; both GC + CCAAT are present. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509 de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727 Steven K Nordeen, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |