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| 产品名称： | HFBBA57 |
|---|---|
| 商品货号： | TS143546 |
| Designations： | HFBBA57 |
| GenBank Number： | M78296 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D13S182E Genomic copy number: unique Gene name: DNA Segment, single copy, expressed Contains complete coding sequence?: U Chromosome: 13; Localization: 13 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.3 Cross references: DNA Seq. Acc.: M78296; EST: EST00444; Primer: M13 Forward Insert lengths(kb): 1.299999952316284 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: DNA Segment, single copy, expressed D13S182E |
| Insert Size (kb)： | 1.300 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8, 0.5; PstI--4.3; HindIII--3.4, 0.9. The insert contains multiple EcoRI fragments (kb): 0.75, 0.5. It is important to note these may have been derived from different cDNAs. This EST has also been mapped to chromosome 8. EST00444 matches GenBank accession CPVATI with 93.90% identity over the sequence range: 322-241. |
| References： | Hawthorn LA, Cowell JK. Regional assignment of EST sequences on human chromosome 13. Cytogenet. Cell Genet. 72: 72-77, 1996. PubMed: 8565640 Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Polymeropoulos MH, et al. Chromosomal distribution of 320 genes from a brain cDNA library. Nat. Genet. 4: 381-386, 1993. PubMed: 8401586 J C Venter, personal communication |