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| 产品名称： | OL202 OL 202 |
|---|---|
| 商品货号： | TS143553 |
| Designations： | OL202 OL 202 |
| Species： | Homo sapiens, human |
| Depositors： | C Wappenschmidt |
| Applications： | Only insert DNA should be used for nick translation. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, MspI, PvuII, TaqI, XbaI. Tested against DNA from 9 unrelated Caucasians. |
| Vector： | Construct size (kb): 5.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.6000000238418579 Tissue: lymphocyte Gene product: DNA Segment, single copy D12S9 Alleles: A2, A1 |
| Insert Size (kb)： | 0.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 0.6; PstI--5.4, 0.45; BamHI--5.8; PvuII--2.9, 2.9; SalI--5.8. Detects fragments of different chromosomal origin; polymorphic bands on chromosome 12, constant bands on chromosome 5, 12, 13, and 17. Codominant segregation demonstrated in 4 informative families with 20 individuals. Digest with BglII gives the following constant bands: 30, 18, 13, 9.6, 8.2, 3.5, 3, 2.6, 2. Only insert DNA should be used for nick translation. Genomic fragments should be separated on 0.9% gels. Washing conditions: 0.1X SSC at 63-65C. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, MspI, PvuII, TaqI, XbaI. Tested against DNA from 9 unrelated Caucasians. |
| References： | Wappenschmidt C, et al. Human cDNA probe detects RFLP on chromosome 12, OL 202 (HMG8 designated no. D12S9). Nucleic Acids Res. 14: 9221, 1986. PubMed: 2878418 |