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微信小章| 产品名称： | pHIR/P12-1 pHP/HIR 12.1 |
|---|---|
| 商品货号： | TS143568 |
| Designations： | pHIR/P12-1 pHP/HIR 12.1 |
| GenBank Number： | X02160 |
| Species： | Homo sapiens, human |
| Depositors： | A Ullrich |
| Vector： | Construct size (kb): 6.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.099999904632568 Tissue: term placenta Gene product: insulin receptor INSR Alleles: A4, D1, D2, A2, B1, B2, E2, S1, S2, S3, A3, C2, E1, C1, A1 |
| Insert Size (kb)： | 4.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.7; PvuII--4.0, 2.5, less than 0.5; HindIII--6.8; SalI--6.8; BamHI--3.6, 3.2. Restriction digests of the clone give the following sizes (kb): BglI--3.7, 1.3, 1.2, 0.78. The insert extends from the EcoRI site at nucleotide 1013 (amino acid 294) through the 3 untranslated region, including poly(A). This insert does not encode the cysteine-rich region which is part of the alpha subunit. The insert does encode a portion of the 3 end of the alpha subunit and all of the beta subunit including the transmembrane and tyrosine kinase domains. A 1.3 kb BglI fragment (12.1B1.3, 1601 - 2963) detects a 3-allele SstI RFLP not detected by the 1.6 kb PstI fragment (12.1P1.6, 2744 - 4343). The BglI fragment encodes the 3 region of the alpha chain and the 5 region of the beta chain. The 7.0/6.2 kb RsaI polymorphism has been detected with the 1.3 kb BglI fragment probe (12.1B1.3, 1601 - 2963). The polymorphic site maps between nucleotides 1928 and 2478 of the cDNA. The 2.4/2.2 kb RsaI polymorphism has been detected with the 1.3 kb BglI fragment (12.1B1.3, nt 1601-2963). The polymorphic site maps between nt 2744 and 2963 of the cDNA. In American Blacks, a 6.4 kb fragment is sometimes (0.6%) detected in RsaI digests. This probably results from another polymorphic RsaI site 0.6 kb from the 7.0 kb fragment. In Pima Indians, a 3.4 kb fragment is detected (34%) in RsaI digests using the 1.3 kb BglI probe. This probably represents another polymorphic site 3.4 kb from one end of both 6.2 and 7.0 kb fragments. The SacI site polymorphism (alleles 9.4 kb/7 + 2.4 kb) appears to be private to Caucasian populations (the 7 + 2.4 kb allele detected in only 1/48 Pima Indians and 1/48 American Blacks). The polymorphic BglII site has been detected using the intact insert or the 1.6 kb PstI fragment (12.1P1.6, 2744-4343). The polymorphic site maps between nucleotides 2744 and 2963 of the cDNA sequence. |
| References： | Patel P, et al. A new Sst 1 RFLP associated with the human insulin receptor locus. Nucleic Acids Res. 16: 5700, 1988. PubMed: 2898769 Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217 Ullrich A, et al. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes. Nature 313: 756-761, 1985. PubMed: 2983222 Elbein SC, et al. Multiple restriction fragment length polymorphisms at the insulin receptor locus: A highly informative marker for linkage analysis. Proc. Natl. Acad. Sci. USA 83: 5223-5227, 1986. PubMed: 3014538 |