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| 产品名称： | pG36/T1 B237/L2-T1 |
|---|---|
| 商品货号： | TS143591 |
| Designations： | pG36/T1 B237/L2-T1 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 21.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 11.0 Gene product: intron processing factor CBP2 |
| Insert Size (kb)： | 11.0 |
| Genotype： | MATalpha leu2-3 leu2-112 cbp2-1 |
| Mating Type： | alpha |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following sites separated by (kb) (approximate): BamHI- 2.4- BamHI- 0.33- EcoRI- 0.55- HindIII. Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--3.9, 3.3, 2.8, 2.3, 1.5, 1.4, 1.0; XhoI--14, 7.8; XbaI--21.7; XbaI/AvaI--7.0, 3.5, 3.2, 2.3, 1.5, 1.4, + others; BamHI--11.0, 4.3, 3.5, 2.3. The insert includes a 1.89 kb open reading frame encoding a 74 kDa basic protein essential for an early step in splicing the cytochrome b pre-mRNA. The probe, pG36/T1, has been deposited in Escherichia coli RR1 as TS143591 and in Saccharomyces cerevisiae B237/L2 as ATCC 66318. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | McGraw P, Tzagoloff A. Assembly of the mitochondrial membrane system. Characterization of a yeast nuclear gene involved in the processing of the cytochrome b pre-mRNA. J. Biol. Chem. 258: 9459-9468, 1983. PubMed: 6348045 |