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| 产品名称： | pAB366Ben |
|---|---|
| 商品货号： | TS143635 |
| Designations： | pAB366Ben |
| Depositors： | BM Tyler |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.1999998092651370 Vector: pAB366Ben (plasmid) Construction: pAB366, pBT6 Marker(s):ampR,bleR,benR Construct size (kb): 7.199999809265137 Features: marker(s): ampR, bleR, benR replicon: pMB1 |
| Applications： | contains sequence tubulin, beta in another host, produces protein tubulin, beta reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5, 0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6, 0.85, 0.55. A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5 and 3 flanking regions. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844 Orbach MJ, et al. Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol. Cell. Biol. 6: 2452-2461, 1986. PubMed: 2946938 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of Escherichia coli containing the plasmid. |