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| 产品名称： | pCOX5bR3.4 |
|---|---|
| 商品货号： | TS143665 |
| Designations： | pCOX5bR3.4 |
| GenBank Number： | M59250 |
| Species： | Homo sapiens, human |
| Depositors： | MI Lomax |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 3.204999923706055 Tissue: fetal liver Gene product: cytochrome c oxidase subunit Vb(cytochrome c oxidase Vb, cytochrome-c oxidase, Vb subunit) COX5B |
| Insert Size (kb)： | 3.205 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 3.0; EcoRI/PvuI--3.2, 1.7, 1.1; PvuII--2.9, 2.5, 0.85; SacI--5.2, 1.3; SacI/EcoRV--3.0, 1.3 (doublet), 0.8; NotI--5.6, 0.8; BamHI--6.6. The insert contains the following restriction sites (bp from the 5 end): EcoRV--2450; NotI--729; PstI--141, 617, 897; PvuII--2661; SacI--1212; SmaI--1340; SphI--499. An RsaI fragment (nt 2014-2224 of the insert, nt 1402-1612 of the published sequence) from intron 4 detects a 15.0 kb HindIII and a 6.4 kb PstI genomic restriction fragment in human DNA, weak HindIII fragments of 4.4 and 3.2 kb in rats, and no hybridizing fragments in Chinese hamsters. Contains sequences beginning 5 to exon to and continuing beyond exon 5. A 1.2 kb SstI/EcoRV fragment (nt 1212-2450 of the insert) detects the following human genomic restriction fragments (kb): COX5BP1-- 3.5 PstI, 15.0 HindIII; COX5BP2--7.9 PstI, 8.4 HindIII; COX5BP3--1.2 PstI, 7.0 HindIII; COX5BP4--3.9 PstI, 4.1 HindIII; COX5BP5--7.6 PstI, 7.0 HindIII; COX5BP6--2.0 PstI, 5.2 HindIII; COX5BP7--6.2 PstI, 16.0 HindIII. Oriented such that the SacI digest gives a restriction fragment of approximately 1.2 kb. Thus the 5 end is adjacent to the T7 promoter. The intact insert can be separated from the vector by a EcoRI/PvuI double digest. |
| References： | Lomax MI, et al. Structure of the human cytochrome c oxidase subunit Vb gene and chromosomal mapping of the coding gene and of seven pseudogenes. Genomics 10: 1-9, 1991. PubMed: 1646156 Margaret I Lomax, personal communication |