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| 产品名称： | AS321 |
|---|---|
| 商品货号： | TS143749 |
| Designations： | AS321 |
| GenBank Number： | M81840 |
| Species： | Homo sapiens, human |
| Applications： | contains sequence DNA Segment, single copy, expressed probes |
| Vector： | Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS- Intact vector size: 2.964 Type of vector: phagemid Vector end: EcoRI Vector end: HindIII Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D14S46E Other gene symbols: NRL; D14S46 Gene name: DNA Segment, single copy, expressed probes Contains complete coding sequence?: Y Tissue: retina, adult Type of DNA: cDNA Insert 5 end: Modification: EcoRI Insert 3 end: Modification: HindIII Insert size (kb): 1.204 Cross references: DNA Seq. Acc.: M81840 Nucleotides 1-1204 of the insert correspond to nucleotides 24-1227 of M81840. Insert lengths(kb): 1.203999996185303 Tissue: retina, adult Gene product: DNA Segment, single copy, expressed probes NRL Target Gene: DNA Segment, single copy, expressed probes |
| Insert Size (kb)： | 1.204 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 1.0, 0.2, EcoRI/KpnI--3.0, 1.2; HindIII--4.0, 0.2; SacI--3.0, 1.2; XhoI--3.7, 0.5. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.96; NcoI--0.005, 0.135, 0.39, 0.44; PstI--0.34, 0.40, 0.65, 1.075; PvuII--0.35, 0.49, 0.55, 0.70; SacI--1.07; XhoI--0.78. In Northern analyses, the insert detects transcripts in retinal tissue of the following species: baboon (1.3 kb); bovine, mouse, and rat (2.0 kb, major); frog (4 kb, 2kb, both faint). |
| References： | Swaroop A, et al. A conserved retina-specific gene encodes a basic motif/leucine zipper domain. Proc. Natl. Acad. Sci. USA 89: 266-270, 1992. PubMed: 1729696 |