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| 产品名称： | pSPMoIL3 MOIL3 |
|---|---|
| 商品货号： | TS143828 |
| Designations： | pSPMoIL3 MOIL3 |
| GenBank Number： | K01668 |
| Species： | Mus musculus, mouse |
| Depositors： | T Yokota |
| Vector： | Construct size (kb): 4.0 |
| Insert： | DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: interleukin 3 (colony-stimulating factor, multiple) Il3 |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains internal PstI, HindIII, BamHI BglII, XbaI, and NcoI sites. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 0.67, 0.48; SacI--4.0; XbaI--3.4, 0.69; SphI/EcoRI--2.45, 1.5; BglII--4.0; PstI--3.1, 0.94. The SphI site in the vector is 432 bp from the SalI cloning site. The SphI/EcoRI fragment contains about 500 bp of non-insert DNA (some from pSP64 and some from pcD). An XhoI fragment from MoIl3 (in a pcD vector) was cloned into the SalI site of pSP64. This fragment contains some of the SV40 promoter and polyadenylation sequences from the pcD vector. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Yokota T, et al. Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proc. Natl. Acad. Sci. USA 81: 1070-1074, 1984. PubMed: 6322187 |