| 产品名称: | pEFD145.1 |
|---|---|
| 商品货号: | TS143965 |
| Designations: | pEFD145.1 |
| Species: | Homo sapiens, human |
| Applications: | TaqI also identifies a 2-allele polymorphism (5.0, 4.1 kb) without a constant band, but this has not yet been verified by family studies. RFLPs observed under normal hybridization and wash stringencies. |
| Vector: | Construct size (kb): 11.19999980926514 |
| Insert: | DNA: genomic Insert lengths(kb): 5.800000190734863 Gene product: DNA Segment, single copy D3S32 Alleles: A1, A2 |
| Insert Size (kb): | 5.800 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 2.7, 2.05, 0.96, 0.96, 0.64, 0.62, 0.6; EcoRI--4.4, 4.4, 2.7; HindIII--4.8, 2.7, 1.55, 1.55, 0.65; BamHI--4.4, 4.4, 2.7; PstI--4.1, 2.8, 1.5, 1.5. The clone contains two copies of the vector. TaqI also identifies a 2-allele polymorphism (5.0, 4.1 kb) without a constant band, but this has not yet been verified by family studies. Codominant segregation of the RsaI RFLP has been demonstrated in 41 3-generation families. RFLPs observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: MspI. |
| References: | Fujimoto E, et al. Isolation and mapping of a polymorphic DNA sequence (pEFD145) on chromosome 3 D3S32. Nucleic Acids Res. 16: 9357, 1988. PubMed: 2902584 |