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| 产品名称： | pIIIEx316 RPR |
|---|---|
| 商品货号： | TS143973 |
| Designations： | pIIIEx316 RPR |
| Depositors： | DR Engelke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8000001907348630 Vector: pIIIEx316 RPR (plasmid) Promoters: Promoter for in vitro transcription T7 Construction: pRS316 Marker(s):URA3,ampR Construct size (kb): 6.800000190734863 Features: insert detection: lacZ marker(s): URA3 marker(s): ampR promoter: RPR1 promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 restriction site: BamHI restriction site: EcoRI restriction site: SalI terminator: RPR1 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector vector permitting RNA synthesis in vitro vector permitting expression of small structured RNAs vector permitting production of single-stranded DNA vector permitting visual detection of recombinants vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; BamHI--6.8; HindIII--6.8. Cloned inserts can be transcribed from the S. cerevisiae RNase P RNA gene (RPR1) intragenic promoter to produce RPR1 leaders fused to RNA corresponding to inserted sequences. One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number. Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS316. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Good PD, Engelke DR. Yeast expression vectors using RNA polymerase III promoters. Gene 151: 209-214, 1994. PubMed: 7828876 |
| Shipped： | freeze-dried |