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微信小章| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品货号： | TS144005 |
| Deposited As： | Clostridium difficile (Hall and OToole) Prevot |
| Strain Designations： | BDMS 18 AN |
| Application： | Quality control strain for Clostridioides difficile agar
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | Toxinotype: 0 |
| Type Strain： | no |
| Comments： | Presence of tcdA and tcdB genes confirmed by PCR
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81). |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Anaerobic |
| Name of Depositor： | Becton Dickinson Microbiology Systems |
| Special Collection： | NCRR Contract |
| Chain of Custody： | ATCC <-- Becton Dickinson Microbiology Systems <-- J Aswell <-- Johns Hopkins Univ. Hosp. Lab |
| References： | Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 |