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| 产品名称： | S-ENDO3.3 |
|---|---|
| 商品货号： | TS144053 |
| Designations： | S-ENDO3.3 |
| GenBank Number： | X72012 |
| Species： | Homo sapiens, human |
| Depositors： | C Bernabeu |
| Vector： | Construct size (kb): 5.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC13 Intact vector size: 2.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI Construction: pBR322, Escherichia coli lacZalpha, M13mp11 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ENG Other gene symbols: HHT1; ORW1; END Gene name: endoglin (Osler-Rendu-Weber syndrome 1) Contains complete coding sequence?: Y Chromosome: 9; Localization: 9 q34.1 Tissue: HL-60 cell line, PMA-treated Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 3.1 Cross references: DNA Seq. Acc.: X72012 Nucleotides 1-3073 of the insert correspond to nucleotides 1-3073 of X72012. Insert lengths(kb): 3.099999904632568 Tissue: HL-60 cell line, PMA-treated Gene product: endoglin (Osler-Rendu-Weber syndrome 1)(Endoglin) END |
| Insert Size (kb)： | 3.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.7; HindIII--5.9; BamHI/MluI--3.0, 2.3, 0.58; BamHI--3.0, 2.9; PstI--4.4, 0.87, 0.68. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--2.7; BglI--3.0; PstI--0.33, 1.04, 1.28. Encodes the S-isoform with a monomeric molecular weight of 85 kDa. This form, which is not the predominant one, includes an exon of 135 bp. |
| References： | Bellon T, et al. Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23: 2340-2345, 1993. PubMed: 8370410 |