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| 产品名称： | pJJ217 |
|---|---|
| 商品货号： | TS144103 |
| Designations： | pJJ217 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Prakash |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 1.764000058174133 Gene product: imidazoleglycerol-phosphate dehydratase HIS3 |
| Insert Size (kb)： | 1.764 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 1.8; PvuII--2.4, 2.0; EcoRI--4.4. BglII and HindIII cut multiple times within the reading frame. A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions. HIS3 is in an orientation opposite to lacZ. The insert was subcloned from pSZ515. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072 |