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微信小章| 产品名称： | pHHH202 |
|---|---|
| 商品货号： | TS144156 |
| Designations： | pHHH202 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Otherwise, RFLPs were observed under normal conditions of hybridization and washing. |
| Vector： | Construct size (kb): 5.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.400000095367432 Gene product: DNA Segment, single copy D17S33 Alleles: A1, A2 |
| Insert Size (kb)： | 2.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.4 doublet, 1.2, 1.0; EcoRI--5.1, 2.4 doublet; HindIII--3.8, 2.4, 1.2; consistent with deposited DNA. The structure of this plasmid is not consistent with the published description of pHHH202. The hybridization pattern of this probe with human DNA was not checked by the ATCC. This plasmid shows some instability. We recommend growing on LB agar with ampicillin, and picking colonies to select the correct clone. Digests also seem to indicate there is a high percentage of vectors without insert present. Linked to von Recklinghausen neurofibromatosis (NF1) (theta = 0.001, LOD score = 12.7). The probe detects genomic HindIII fragments of 2.4 and 1.6 kb and does not cross-hybridize to rat or mouse sequences. Codominant segregation demonstrated in 49 3-generation families for the RsaI RFLP. This probe was pre-associated with excess human DNA before hybridization. Otherwise, RFLPs were observed under normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, MspI, PstI, PvuII, TaqI. |
| References： | OConnell P, et al. Two NF1 translocations map within a 600-kilobase segment of 17q11.2. Science 244: 1087-1088, 1989. PubMed: 2543077 Leach RJ, et al. Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. Genomics 5: 167-176, 1989. PubMed: 2571568 Ward K, et al. Diagnosis of neurofibromatosis I by using tightly linked flanking DNA markers. Am. J. Hum. Genet. 46: 943-949, 1990. PubMed: 1971145 Hoff M, et al. Isolation and mapping of a polymorphic DNA sequence pHHH202 on chromosome 17 D17S33. Nucleic Acids Res. 16: 781, 1988. PubMed: 2893349 Skolnick MH, et al. Linkage of NF1 to 12 chromosome 17 markers: a summary of eight concurrent reports. Genomics 1: 382-383, 1987. PubMed: 2896634 |