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| 产品名称： | lambdaD18 pcD-GALT |
|---|---|
| 商品货号： | TS144175 |
| Designations： | lambdaD18 pcD-GALT |
| GenBank Number： | M12950 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc, TH Rabbitts |
| Vector： | Construct size (kb): 45.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 16.20000076293945 Tissue: D-PLL cell line (B-prolymphocytic leukemia) Gene product: T cell receptor, gamma, joining segment JP1 TCRGC1 |
| Insert Size (kb)： | 16.200 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 11.0, 9.2, 4.4, 1.7, 1.25; SacI--20.0, 9.2, 7.0, 4.4 (doublet), 1.2, 0.9; XhoI--20.0, 9.2, 9.0, 7.8, 1.25; SmaI--20.0, 13.5, 5.8, 4.6, 2.5; PvuI--23.0, 12.0, 8.2; HindIII--23.0, 14.0, 2.7, 2.2, 1.4. The insert contains the following restriction sites (approximate kb from the 5 BamHI site): HindIII--3.5, 4.8, 6.3, 8.6, 10.3, 12.4, 15.2. Contains germline DNA beginning about 3.6 kb upstream of JgammaP1 and extending about 2.8 kb beyond exon 1 of Cgamma1. Additional GenBank/EMBL accession numbers: M12960, M14996, X03434, X08084. |
| References： | Lefranc MP, Rabbitts TH. Two tandemly organized human genes encoding the T-cell gamma constant-region sequences show multiple rearrangement in difference T-cell types. Nature 316: 464-466, 1985. PubMed: 2991773 Lefranc MP, et al. Genetic polymorphism and exon changes of the constant regions of the human T-cell rearranging gene gamma. Proc. Natl. Acad. Sci. USA 83: 9596-9600, 1986. PubMed: 2879283 Marie-Paule Lefranc, personal communication |