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| 产品名称： | pRX-3 |
|---|---|
| 商品货号： | TS144182 |
| Designations： | pRX-3 |
| Depositors： | TD Pollard |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.8499999046325680 Vector: pRX-3 (plasmid) Promoters: Promoter trpE Construction: pBR322, trpE, M13mp13 polylinker Marker(s):ampR Construct size (kb): 2.849999904632568 Features: marker(s): ampR promoter: trpE replicon: pMB1 MCS: EcoRI...ClaI terminator: unknown terminator |
| Applications： | expression vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.85; ClaI--2.85. When Escherichia coli CAG-456 (a protease deficient host) is used, this vector yields large amounts of the fusion protein (100 mg/liter or 45% of the soluble protein). One of a series of plasmid expression vectors (ATCC 37677 to 37679) representing multiple reading frames for expression from the trpE promoter. The fusion proteins produced contain less than 25 amino acids of the trpE protein. This vector contains the trpE operon and 51 bp of the trpE gene, followed by a 12-mer EcoRI linker and the M13mp13 multiple cloning site (MCS). There is a TGA stop codon in frame from the GAA of the EcoRI site 33 bp beyond the MSC ClaI site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Rimm DL, Pollard TD. New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli. Gene 75: 323-327, 1989. PubMed: 2653968 |
| Related Products： | component of:ATCC 87599 |
| Shipped： | freeze-dried |