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| 产品名称： | pMNS01A |
|---|---|
| 商品货号： | TS144248 |
| Designations： | pMNS01A |
| GenBank Number： | D12536 M29671 |
| Species： | Melon necrotic spot virus, Melon necrotic spot virus |
| Depositors： | DM Rochon |
| Applications： | contains sequence genome, partial, 3 end |
| Vector： | Construct size (kb): 4.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.150000095367432 Gene product: genome, partial, 3 end Target Gene: genome, partial, 3 end |
| Insert Size (kb)： | 2.150 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.6, 2.4 (partial); HindIII--3.4, 1.5; BamHI--3.2, 1.35, 0.35. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.2, 1.55; BglII--1.0; ClaI--1.7; EcoRI--0.1; EcoRV--1.95; HindIII--0.75; KpnI--1.8; NcoI--0.3, 1.45; SalI--1.4; SmaI--0.6, 1.6. Virus was isolated from cucumber, Cucumis sativus. The insert hybridizes to the genomic length RNA (4.26 kb) and two shorter RNAs of about 1.9 kb and 1.6 kb. |
| Classification： | Carmovirus |
| References： | Riviere CJ, Rochon DM. Nucleotide sequence and genomic organization of melon necrotic spot virus. J. Gen. Virol. 71: 1887-1896, 1990. PubMed: 2212985 DAnn M Rochon, personal communication |