| 产品名称: | pUC18T25 |
|---|---|
| 商品货号: | TS144284 |
| Designations: | pUC18T25 |
| GenBank Number: | D01101 |
| Species: | Tomato aspermy virus (TAV) |
| Depositors: | F Garcia-Arenal |
| Vector: | Construct size (kb): 4.210000038146973 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.509999990463257 |
| Insert Size (kb): | 1.510 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Restriction digests of the clone give the following sizes (kb): HincII--4.2; HindIII--3.1, 1.15; PvuII--2.4, 0.95 (doublet); XhoI--2.7, 1.4; SmaI--4.2; EcoRI/PstI--2.6, 1.6. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.44; PvuII--0.71; ClaI--0.89, 1.36; XhoI--0.95; SmaI--1.39. Contains nucleotides 1893-3402, which includes the 3 end of the ORF and the 3 untranslated region. |
| Classification: | Cucumovirus |
| References: | Bernal JJ, et al. Evolutionary relationships in the cucumoviruses: nucleotide sequence of tomato aspermy virus RNA 1. J. Gen. Virol. 72: 2191-2195, 1991. PubMed: 1895056 |