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| 产品名称： | Human respiratory syncytial virus |
|---|---|
| 商品货号： | TS144325 |
| Classification： | Paramyxoviridae, Pneumovirus |
| Deposited As： | Respiratory syncytial virus |
| Agent： | Human respiratory syncytial virus |
| Strain： | A2 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | VR-1540 was prepared from ATCC VR-1302; by growth in the presence of neutralizing serum to remove contaminating Human adenovirus 1. |
| Effect on Host： | CPE, syncytial formation, rounding, and sloughing |
| Recommended Host： | HEp-2 (ATCC CCL-23) |
| Growth Conditions： | Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | L Potash, Flow Laboratories, Inc.; ATCC |
| Special Collection： | NCRR Contract |
| Source： | Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961 |
| References： | Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423 Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961. |