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微信小章| 产品名称： | EY2 lambdaLASEY2 |
|---|---|
| 商品货号： | TS144451 |
| Designations： | EY2 lambdaLASEY2 |
| Species： | Homo sapiens, human |
| Depositors： | A Swaroop |
| Origin： | eye cell line |
| Vector： | Tissue: eye, cell line CRL-7475 Type of insert: cDNA Insert size range: > 0.8 kb Genome Equivalent: 0.0 Genome: human Vector : Charon BS- Insert size range (kb) : > 0.8 kb Vector ends : Modification : EcoRI HindIII Number of independent recombinants : 1.0 x 106 Titer : 1.3 x 108 pfu/ml |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen bacteria-free phage lysate. |
| Comments： | cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5GCTTGAATTCAAGC 3) was added for directional cloning. |
| References： | Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993. Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057 Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160 |