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微信小章| 产品名称： | pEX2TBP |
|---|---|
| 商品货号： | TS144529 |
| Designations： | pEX2TBP |
| GenBank Number： | M25318 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 2.839999914169312 |
| Insert： | DNA: genomic Insert lengths(kb): 0.136000007390976 Gene product: T cell receptor, gamma, constant region C2 TCRGC2 Alleles: B1, D4, D2, A1, A2, B2, D1, D3 |
| Insert Size (kb)： | 0.136 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.14; EcoRI--2.84; HindIII--2.84; PstI--2.84; BamHI--2.84. Subcloned from lambdaR12. Contains the triplicated exon 2 (ex2T). Cross hybridizes to exon2 of TCRGC1 and to different exons of TCRGC2 (ex2R, ex2). For the HindIII polymorphism, the 2.7 and 4.1 kb constant bands correspond to TCRGC1 and the 15 kb constant band corresponds to the 5 region of TCRGC2. In EcoRI digests, the 2.3 kb band corresponds to ex2 of TCRGC1, the 1.4 kb band to TCRGC2 ex2R, and the 1.6 band to TCRGC2 ex2. In PvuII digests, the 9.4 kb band corresponds to the TCRGC1 gene. |
| References： | Buresi C, et al. Exon duplication and triplication in the human T-cell receptor gamma constant region genes and RFLP in French, Lebanese, Tunisian, and Black African populations. Immunogenetics 29: 161-172, 1989. PubMed: 2564370 Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738 Marie-Paule Lefranc, personal communication |