宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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Fr8-77

货号 TS144550
中文名称 null
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产品简介
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产品名称: Fr8-77
商品货号: TS144550
Designations: Fr8-77
Species: Homo sapiens, human
Vector:
Construct size (kb): 4.5
Insert:
DNA: genomic
Insert lengths(kb): 1.5
Gene product: DNA Segment, single copy D21S82
Alleles: A3, B2, A1, A2, B1, B3
Insert Size (kb): 1.5
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; HindIII--4.4; EcoRI/HindIII--3.0, 1.45; BglI--4.4; PvuII--4.4.
Insert can be labeled by transcription with SP6 polymerase on HindIII linearized plasmid.
Codominant segregation shown in 2 informative families (10 individuals).
The locus D21S82 is very closely linked to the locus SOD1 on chromosome 21.
No background at stringency of 0.2X SSC, 65C.
Enzyme(s) not detecting polymorphism: BglII, HindIII, MspI, PstI, RsaI and TaqI, in 5 unrelated Caucasians.
References:

Xiao GH, et al. Anonymous DNA sequence from chromosome 21 showing a three allele insertion/deletion RFLP (HGM 9 provisional no. D21S82). Nucleic Acids Res. 15: 5499, 1987. PubMed: 2885809

Warren AC, et al. A genetic linkage map of 17 markers on human chromosome 21. Genomics 4: 579-591, 1989. PubMed: 2568330