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| 产品名称： | pRS404 |
|---|---|
| 商品货号： | TS144557 |
| Designations： | pRS404 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.2740001678466800 Vector: pRS404 (phagemid) Promoters: Promoter T7 Construction: pBluescript II KS+ Marker(s):TRP1,ampR Construct size (kb): 4.27400016784668 Features: insert detection: lacZ marker(s): TRP1 marker(s): ampR promoter: T3 promoter: T7 promoter: lac replicon: f1 replicon: pMB1 MCS: KpnI...SacI |
| Applications： | YI-type (integrating) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.35, 0.95; BglI--2.6, 1.7; PvuII--3.85, 0.45. A YI-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 |
| Related Products： | component of:ATCC 87532 component of:ATCC 87536 |
| Shipped： | frozen |