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| 产品名称： | pHf0.2 HF0.2 |
|---|---|
| 商品货号： | TS144608 |
| Designations： | pHf0.2 HF0.2 |
| Species： | Homo sapiens, human |
| Depositors： | NA Ellis |
| Applications： | Sequence analysis indicates a small amount of Alu repeat sequence on the probe. |
| Vector： | Construct size (kb): 3.009999990463257 |
| Insert： | DNA: genomic Insert lengths(kb): 0.1899999976158142 Tissue: DNA from 853 hybrid cell line (Y only) Gene product: pseudoautosomal boundary region, X-linked PABX Alleles: A1, A2, |
| Insert Size (kb)： | 0.190 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0; BamHI--3.0; EcoRI/BamHI--2.8, 0.20; PstI--3.0; HindIII--3.0. The pseudoautosomal boundary lies proximal to the sequences defined by pHf0.2 between the EcoRI and RsaI sites. In RsaI digests, detects 2.4 and 2.8 kb bands in males, but only 2.8 kb band in females (2.4 kb band is Y-specific, 2.8 kb band is X-specific). DNA from male and female humans, gorillas and chimpanzees show fragments of identical sizes. Hybridizes to 1.8 kb band in EcoRI digests, and 0.6 kb in HaeIII digests for both males and females. Detects 3.3 and 4.5 kb bands in male SacI but only 4.5 kb in female SacI digests (3.3 kb band is Y-specific, 4.5 kb is X-specific). Sequence analysis indicates a small amount of Alu repeat sequence on the probe. The polymorphic MspI site detected by this probe lies between the probe sequences and the pseudoautosomal boundary, 181 bp from a non-polymorphic MspI site in the pseudoautosomal region. |
| References： | Ellis NA, et al. The pseudoautosomal boundary in man is defined by an Alu repeat sequence inserted on the Y chromosome. Nature 337: 81-84, 1989. PubMed: 2909893 Ellis N, et al. Strong linkage disequilibrium between the XY274 polymorphism and the pseudoautosomal boundary. Am. J. Hum. Genet. 46: 950-955, 1990. PubMed: 2339693 Michael Nerenberg, personal communication |