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| 产品名称： | pMCT15 RW-139 |
|---|---|
| 商品货号： | TS144622 |
| Designations： | pMCT15 RW-139 |
| Species： | Homo sapiens, human |
| Applications： | Subcloned from cMCT15 isolated by YN-12 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC). |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: genomic Insert lengths(kb): 2.700000047683716 Gene product: DNA Segment, single copy D21S113 |
| Insert Size (kb)： | 2.700 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): TaqI--0.75, 0.5 + smaller; BamHI--5.5; EcoRI--5.5; HindIII--5.5; PvuII--2.3, 1.55, 0.6, 0.55, 0.4. The MspI polymorphism demonstrates codominant segregation in 40 three generation families. Subcloned from cMCT15 isolated by YN-12 oligonucleotide; RFLPs observed under normal stringency (0.1X SSC). Enzyme(s) not detecting polymorphism: RsaI, HindIII. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pMCT15) on chromosome 21 D21S113. Nucleic Acids Res. 16: 9882, 1988. PubMed: 2903484 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 |