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微信小章| 产品名称： | pSM-1 UTR-00282, WI-8985 |
|---|---|
| 商品货号： | TS144648 |
| Designations： | pSM-1 UTR-00282, WI-8985 |
| GenBank Number： | X02744 |
| Species： | Homo sapiens, human |
| Applications： | A 2.0 kb BamHI fragment was used to detect the RFLP. |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.599999904632568 Tissue: HuT-102 cell line (adult T-cell leukemia) Gene product: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)(platelet-derived growth factor, beta polypeptide) SIS Alleles: B1, B2, B1, B2 |
| Insert Size (kb)： | 2.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 2.1, 0.68; SalI--5.8; HindIII--5.8; PstI--4.6, 1.35; BglII--5.8. The insert includes 2498 bp followed by 92 adenylates. The insert is located downstream from a SV40 enhancer, early promoter and 16S and 19S mRNA splice sites, and upstream from SV40 poly(A) signal. The insert contains a BamHI site. The 2.0 kb BamHI fragment contains all the coding sequences of the mRNA, plus some pcD sequence at the 5 end. A 2.0 kb BamHI fragment was used to detect the RFLP. |
| References： | Clarke MF, et al. Transformation of NIH 3T3 cells by a human c-sis cDNA clone. Nature 308: 464-467, 1984. PubMed: 6323994 Josephs SF, et al. Transforming potential of human c-sis nucleotide sequences encoding platelet-derived growth factor. Science 225: 636-639, 1984. PubMed: 6740330 Ratner L, et al. Nucleotide sequence of transforming human c-sis cDNA clones with homology to platelet-derived growth factor. Nucleic Acids Res. 13: 5007-5018, 1985. PubMed: 2991848 |