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| 产品名称： | Hepatitis A virus |
|---|---|
| 商品货号： | TS144724 |
| Classification： | Picornaviridae, Hepatovirus |
| Agent： | Hepatitis A virus |
| Strain： | HM 175 (clone 1) |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Effect on Host： | No CPE expected. CPE at high titer, cell rounding, sloughing, degeneration |
| Recommended Host： | FRhK4 (ATCC® CRL-1688x99) |
| Growth Conditions： | Temperature: 34.5°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34.5°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 14-28 days |
| Name of Depositor： | RH Purcell, National Institute of Allergy and Infectious Diseases; ATCC |
| Special Collection： | NCRR Contract |
| Source： | Human stool specimens from an outbreak of the virus, Australia. |
| References： | Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685 Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985 |