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微信小章| 产品名称： | Human Parechovirus 2 |
|---|---|
| 商品货号： | TS144823 |
| Classification： | Picornaviridae, Parechovirus |
| Deposited As： | Echovirus 23 |
| Agent： | Human Parechovirus 2 |
| Strain： | Williamson |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation from primary cell culture product ATCC® VR-53™ to HT-29 cells (ATCC® HTB-38™). ATCC® VR-53™ and ATCC® VR-1820™ have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture. |
| Effect on Host： | CPE, rounding and sloughing |
| Recommended Host： | HT-29 cells (ATCC® HTB-38™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 65-75% confluent, 18-24 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | HA Wenner |
| Source： | Rectal swab from child with diarrhea, Ohio, 1956 |
| References： | Wenner HA, Lenahan MF. Propagation of group A Coxsackie viruses in tissue cultures. II. Some interactions between virus and mammalian cells. Yale J. Biol. Med. 34: 421-438, 1961. PubMed: 14006144 Westerhuis BM, et al. Growth characteristics of human parechovirus 1 to 6 on different cell lines and cross- neutralization of human parechovirus antibodies: a comparison of the cytopathic effect and real time PCR. Virol. J. 10: 146, 2013. PubMed: 23668373 Wigand R, Sabin AB. Properties of ECHO types 22, 23 and 24 viruses. Arch. Gesamte Virusforsch. 11: 224-247, 1961. PubMed: 13785166 |