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| 产品名称： | pPLc28 PL-C, pST28 |
|---|---|
| 商品货号： | TS144854 |
| Designations： | pPLc28 PL-C, pST28 |
| Depositors： | Immunex Corporation, S Gillis, Immunex Corporation |
| Applications： | expression vector |
| Vector： | Construct size (kb): 2.72000002861023 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PstI--2.7; BglI--2.7; HindIII--2.7; BamHI--2.7. The following unique restriction sites are found on this vector separated by (bp)(approx): BglI- 100- PstI- 150- PvuI- 1020- EcoRI- 10- BamHI-HindIII-1420. This vector is used for expression of fragments containing a ribosome binding site. The orientation of the PL promoter is clockwise with respect to the plasmid ori. |
| References： | Remaut E, et al. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene 15: 81-93, 1981. PubMed: 6271633 Anderson DM, et al. DNA sequences encoding bovine interleukin-2. US Patent 4,882,282 dated Nov 21 1989 Namen AE, et al. Interleukin-7. US Patent 4,965,195 dated Oct 23 1990 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |