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微信小章| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS144922 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | MG1655 |
| Application： | Transformation host Bacteriophage host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Shipping Information： | Frozen gycerol stock of E. coli |
| Storage Conditions： | -80°C or colder |
| Preceptrol®： | no |
| Genotype： | F- lambda- |
| Type Strain： | no |
| Comments： | Electronic access to the correlations between the restriction map and the ordered phage library of Kohara are available over the Internet by anonymous ftp at these hosts: ncbi.nlm.nih.gov and utmmg.med.uth.tmc.edu. Host strain with well-characterized restriction maps for NotI, SfiI, XbaI and BlnI. Can be used as a molecular size marker for pulsed-field gel electrophoresis. AE000111 through AE000510 are individual GenBank numbers for the complete sequence, U00096 Derived from the parent strain W1485 by curing of the F plasmid. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | GM Weinstock, University of Texas Medical School |
| Chain of Custody： | ATCC |
| References： | Blattner FR. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 21: 5408-5417, 1993. PubMed: 8265357 Blattner FR, et al. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462, 1997. PubMed: 9278503 Burland V, et al. DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics 16: 551-561, 1993. PubMed: 7686882 Daniels DL, et al. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science 257: 771-778, 1992. PubMed: 1379743 Dicker IB. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173: 334-344, 1991. PubMed: 1987124 Heath JD, et al. NotI genomic cleavage map of Escherichia coli K-12 strain MG1655. J. Bacteriol. 174: 558-567, 1992. PubMed: 1729244 Perkins JD, et al. XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains. J. Mol. Biol. 232: 419-445, 1993. PubMed: 8393935 Plunkett G III, et al. Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 21: 3391-3398, 1993. PubMed: 8346018 Johanson U, Hughes D. Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli. Gene 120: 93-98, 1992. PubMed: 1398129 Jensen KF. The Escherichia coli K-12 wild types W3110 and MG1655 have an rphframeshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. Gene 175: 3401-3407, 1993. PubMed: 8501045 Freddolino PL, Amini S, and S Tavazoie. Newly Identified Genetic Variations in Common Escherichia coli MG1655 Stock Cultures. J. Bacteriol. 194(2): 303-306, 2012. PubMed: 22081388 |
| Cross References： | Nucleotide (GenBank) : U00096 complete sequence Nucleotide (GenBank) : X72920 intercistronic region Nucleotide (GenBank) : X64592 gene for ribosomal protein S7, partial Nucleotide (GenBank) : L10328 chromosomal region from 81.5 to 84.5 minutes Nucleotide (GenBank) : L19201 chromosomal region from 87.2 to 89.2 minutes Nucleotide (GenBank) : M87049 chromosomal region from 84.5 to 86.5 minutes Nucleotide (GenBank) : U00006 chromosomal region from 89.2 to 92.8 minutes Nucleotide (GenBank) : X54797 firA gene, firA(200) and firA(201) allele, and skp gene (3 end) |