微信小陈
微信小章
产品简介
购买须知
| 产品名称： | YCplac33 |
|---|---|
| 商品货号： | TS144953 |
| Designations： | YCplac33 |
| Depositors： | RD Gietz |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.6030001640319820 DESCRIPTION OF VECTOR: Intact vector size: 5.603 Type of vector: plasmid Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Other unique sites: EcoRV NdeI AatII NarI SpeI NaeI NheI BglII ClaI NsiI StuI Construction: pUC19 Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): promoter: lac, ->, 143-171 insert detection: lacZ, ->, 216-500 MCS: HindIII...EcoRI, ->, 234-285 centromere: CEN4, 504-1480 replicon: ARS1, 1481-2225 marker(s): URA3, marker(s): ampR, ->, 3802-4662 replicon: pMB1, 5420-5420 Vector: YCplac33 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 5.603000164031982 Features: insert detection: lacZ marker(s): URA3 marker(s): ampR promoter: lac replicon: ARS1 replicon: pMB1 MCS: HindIII...EcoRI centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): AvaI--3.7, 1.9; ClaI--5.7; PstI--5.6. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106 |
| Related Products： | component of:ATCC 87623 component of:ATCC 87638 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |