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微信小章| 产品名称： | Human herpesvirus 5 |
|---|---|
| 商品货号： | TS144992 |
| Classification： | Herpesviridae, Cytomegalovirus |
| Deposited As： | Cytomegalovirus |
| Agent： | Human herpesvirus 5 |
| Strain： | Davis |
| Common Name： | Human cytomegalovirus |
| Applications： | Sexually Transmitted Disease Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Liver biopsy, 3-month-old girl with microencephaly and other symptoms resembling congenital toxoplasmosis |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Images： |  |
| Comments： | Virus primarily cell-associated. For each passage, perform freeze-thaws to facilitate viral release. A long (10-11 day) incubation is required for initial passage of frozen stocks. Add contents of ampule to a 80-90% monolayer of cells. For preservation, add FBS to virus culture to produce a final 20% FBS concentration. |
| Effect on Host： | CPE, focal or generalized cytopathology with rounding, cytomegaly, and intranuclear inclusions |
| Recommended Host： | MRC-5 cells (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | T Weller |
| Special Collection： | NCRR Contract |
| Source： | Liver biopsy, 3-month-old girl with microencephaly, and other symptoms resembling congenital toxoplasmosis |
| References： | Weller TH, et al. Isolation of intranuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proc. Soc. Exp. Biol. Med. 94: 4-12, 1957. PubMed: 13400856 Weller TH, et al. Serologic differentiation of viruses responsible for cytomegalic inclusion disease. Virology 12: 130-132, 1960. PubMed: 13843861 |
| Cross References： | Nucleotide (GenBank) : JX512198.1 Human herpesvirus 5 strain Davis, complete genome |