微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pSV2-gpt |
|---|---|
| 商品货号： | TS145014 |
| Designations： | pSV2-gpt |
| Depositors： | P Berg |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 5.50
Vector: pSV2-gpt (plasmid)
Promoters: Promoter SV40
Construction: SV40, pBR322, Escherichia coli
Marker(s): ampR,gpt+
Construct size (kb): 5.5
Features: marker(s): ampR, gpt+ promoter: SV40 replicon: pMB1 terminator: SV40 early |
| Applications： | Expression vector
Shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/BglII--5.2, 0.12; HindIII--5.2; HindIII/EcoRV--4.7, 0.58; PvuII--5.3; PstI--4.3, 0.9; PvuII/PstI--2.8, 1.5, 0.9. Plasmid containing cells use xanthine as a guanine nucleotide precursor. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549 Mulligan RC, Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 78: 2072-2076, 1981. PubMed: 7017722 |
| Shipped： | frozen |