微信小陈
微信小章
产品简介
购买须知
| 产品名称： | YEp426 pRB687 |
|---|---|
| 商品货号： | TS145045 |
| Designations： | YEp426 pRB687 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.1999998092651400 Vector: YEp426 (plasmid) Construction: YEp425, pBR322 Marker(s):ampR,LYS2 Construct size (kb): 11.19999980926514 Features: marker(s): ampR, LYS2 replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.4, 3.8; BamHI/HindIII--7.0, 3.8, 1.4; ClaI/SalI--6.8, 6.0. YE-type shuttle vector. The order of the major features in this plasmid is: ClaI site - LYS2 - SalI site - ori(2 micron) - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185 |
| Shipped： | freeze-dried |