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| 产品名称： | pBP111 |
|---|---|
| 商品货号： | TS145047 |
| Designations： | pBP111 |
| Depositors： | RH Reeves |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.6000003814697270 Vector: pBP111 (plasmid) Promoters: Promoter lac Construction: YTCA-1x, pRS303, pL1.1A Marker(s):ampR,HIS3 Construct size (kb): 8.600000381469727 Features: marker(s): ampR, HIS3 promoter: lac, SP6 replicon: pMB1 |
| Applications： | acentric fragmentation vector shuttle vector vector permitting RNA synthesis in vitro |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--4.3 (doublet); EcoRI--7.0, 1.6; SacI--4.6, 4.0; BamHI--4.7, 2.8, 1.2. Acentric chromosome fragmentation vector targeting LINE sequences. Contains promoters for in vitro RNA synthesis. Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation. The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site. Constructed from pBP103 (ATCC 77087) by inserting a 4.0 kb LINE-containing SacI fragment from pL1.1A into the SacI site. pBP110 (ATCC 77090) and pBP111 (TS145047) differ in the orientation of the LINE sequence. The order of the major features in this plasmid is: bla - HIS3 - lacZ/MCS/3LINE5 - HindIII - SphI - PstI - AccI - SalI - TEL - ClaI - EcoRV - SP6 promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Pavan WJ, et al. High-efficiency yeast artificial chromosome fragmentation vectors. Gene 106: 125-127, 1991. PubMed: 1937033 Reeves RH, et al. Yeast artificial chromosome modification and manipulation. Methods Enzymol. 216: 584-603, 1992. PubMed: 1336105 |
| Shipped： | freeze-dried |