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| 产品名称： | lambdaSP19 |
|---|---|
| 商品货号： | TS145100 |
| Designations： | lambdaSP19 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc |
| Vector： | Construct size (kb): 44.09999847412109 |
| Insert： | DNA: genomic Insert lengths(kb): 14.60000038146973 Tissue: T-lymphocyte SP-F7 cell line Gene product: T cell receptor, gamma, variable region V5 TCRGC1 |
| Insert Size (kb)： | 14.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--19.0, 13.0, 6.4, 4.6; BglII--27.0, 8.0, 7.5; EcoRI--23.0, 10.0, 3.4, 2.8, 2.5; BamHI--22.0, 20.0; HindIII--22.0, 11.5 (doublet); PvuI--20.0, 15.0, 8.5; SacI--20.0, 9.4, 8.5, 4.4. The insert contains the following restriction sites (approximate kb from the 5 end): KpnI--1.65, 5.4, 5.8, 12.1; EcoRI--3.05, 6.0, 8.3, 11.0; HindIII--3.15, 3.6, 7.8, 12.5; BamHI--14.6. The 3 end of this insert is the naturally occurring BamHI site in C1 exon 1. Contains germline V5, germline V4, the 5 end of C1 exon 1, and an unusual rearrangement abutting the V3 recombination signal to J1 in an inverted orientation. lambdaSP14 (ATCC 65836, 65837) and lambdaSP19 (TS145100, 65839) are end clones from the same inversion (47 kb) event. |
| References： | Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738 |