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| 产品名称： | pUC-RI-4large 0173 |
|---|---|
| 商品货号： | TS145130 |
| Designations： | pUC-RI-4large 0173 |
| GenBank Number： | X02159 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 3.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8209999799728394 Tissue: HL-60 cell line (promyelocytic leukemia) Gene product: tumor necrosis factor, alpha (cachectin) TNFA |
| Insert Size (kb)： | 0.821 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.5; BglI--2.0, 1.2, 0.4; EcoRI--2.7, 0.9; HindIII--3.5; PvuII--2.4, 0.55, 0.4, 0.25. The insert includes the following restriction sites (approximate kb from the 5 end): PvuII--0.18, 0.42; HindIII--0.64. Constructed from lambda 42-4 and lambda 16-4. The insert begins in the translated region and extends to the naturally occurring EcoRI site at nt 1070 in the 3 untranslated region. Contains the complete coding sequence for the mature protein, but lacks the 5 portion of the signal peptide coding sequence. Also contains part of the 3 untranslated region. A 578 bp AvaI/HindIII fragment encodes amino acids 8-157. |
| References： | Pennica D, et al. Human tumor necrosis factor: precursor structure, expression, and homology to lymphotoxin. Nature 312: 724-729, 1984. PubMed: 6392892 |