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| 产品名称： | Infectious pancreatic necrosis virus |
|---|---|
| 商品货号： | TS145166 |
| Classification： | Birnaviridae,Aquabirnavirus |
| Agent： | Infectious pancreatic necrosis virus |
| Strain： | LWVRT 60-1 |
| Applications： | Replication of the virus has not been observed in amphibian or mammalian cell lines. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Rainbow trout (Salmo gairdneri), West Virginia, 1960 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Brook trout 4 weeks of age become ill and die 1 to 4 weeks after feeding the virus. Antigenic for Salmo gairdneri, Salvelinus frontinalis, goats and rabbits. Replication of the virus has not been observed in amphibian or mammalian cell lines. This is a serious salmonid fish pathogen. It apparently originated in North America but has since been introduced to Europe and Japan. Stable at -20C and in 50% glycerol at 4C. |
| Effect on Host： | Yes, in vitro effects: cytopathic effect, refractile rounding CPE; refractile rounding |
| Recommended Host： | Recommended Host:xa0 RTG-2 (ATCC® CCL-55TM) |
| Growth Conditions： | Temperature: 18.0°C Duration: 1 to 2 days; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent). |
| Name of Depositor： | KE Wolf, MC Quimby |
| Special Collection： | ATCC |
| Source： | Rainbow trout (Salmo gairdneri), West Virginia, 1960 |
| Year of Origin： | 1960 |
| References： | Wolf K, Quimby MC, Bradford AD. Egg-associated transmission of IPN virus of trouts. Virology 21: 317-321, 1963. Snieszko SF, et al. Virus nature of infectious pancreatic necrosis in trout. Proc. Soc. Exp. Biol. Med. 104: 105-108, 1960. PubMed: 13845654 can be grown on other fish cell lines; salmonid fishes (Salvelinus fontinalis, Salmo gairdneri, S. trutta, S. clarki, Salvelinus namaycush, Oncorhynchus kisutch, Salmo salar) |