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微信小章| 产品名称： | 90.74 |
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| 商品货号： | TS145184 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Cell Type： | transformed with adenovirus 5 DNA |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Human adenovirus sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus |
| Applications： | packaging cell line |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | This line is an amphotropic retrovirus packaging cell line derived from the human embryonic kidney line, 293 (ATCC CRL-1573). 293 cells were co-transfected with the pIK6.1MCVampac plasmid (ATCC 75483) encoding the gag and pol genes from ectropic MMLV and the envelope gene from the 4070A amphotropic MLV and MC1 neo U.S. Pat. 5,858,740. The line does not depend on continued G418 selection to maintain the packaging genome over time. |
| Comments： | This line is an amphotropic retrovirus packaging cell line derived from the human embryonic kidney line, 293 (ATCC CRL-1573). 293 cells were co-transfected with the pIK6.1MCVampac plasmid (ATCC 75483) encoding the gag and pol genes from ectropic MMLV and the envelope gene from the 4070A amphotropic MLV and MC1 neo U.S. Pat. 5,858,740. The line does not depend on continued G418 selection to maintain the packaging genome over time. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 22 hours |
| Name of Depositor： | Cell Genesys |
| U.S. Patent Number： | |
| References： | Finer MH, et al. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells. US Patent 5,858,740 dated Jan 12 1999 Finer MH, et al. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells. US Patent 6,506,604 dated Jan 14 2003 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |