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| 产品名称： | pODC821 |
|---|---|
| 商品货号： | TS145186 |
| Designations： | pODC821 |
| GenBank Number： | X07944 |
| Species： | Rattus norvegicus, rat |
| Depositors： | CT Van Oostrom, H van Steeg |
| Applications： | in suitable host, produces protein ornithine decarboxylase 1 |
| Vector： | Construct size (kb): 10.44999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 7.776000022888184 Tissue: liver Gene product: ornithine decarboxylase 1 ODC1 Target Gene: ornithine decarboxylase 1 |
| Insert Size (kb)： | 7.776 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.8, 2.7; PstI--4.2, 3.8, 1.0, 0.65, 0.5, 0.3; EcoRI--5.3, 5.2; HindIII--4.9, 2.6, 2.4, 0.6; SalI--10.5. The insert contains the following restriction sites (nt from the 5 end): PstI--1170 (exon 1), 1603 (intron 1), 5820 (exon 10), 6892, 7523 (exon 12); HindIII--2313 (intron 1), 4935 (exon 8), 7206 (exon 12); EcoRI--5207 (intron 8). Contains the complete gene (12 exons) with 5 and 3 flanking sequences. When the insert is cloned into pSV2neo and transfected into enzyme-deficient CHO cells, the enzyme is synthesized and is inducible by insulin or tetradecanoyl phorbol acetate. |
| References： | van Steeg H, et al. Cloning and functional analysis of the rat ornithine decarboxylase-encoding gene. Gene 93: 249-256, 1990. PubMed: 2227438 |