宁波泰斯拓生物

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p2A7

货号 TS145189
中文名称 null
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产品简介
购买须知
产品名称: p2A7
商品货号: TS145189
Designations: p2A7
Species: Homo sapiens, human
Vector:
Construct size (kb): 5.400000095367432
Insert:
DNA: cDNA
Insert lengths(kb): 2.700000047683716
Tissue: placenta
Gene product: steroid sulfatase (microsomal)(steroid sulfatase, microsomal) STS
Insert Size (kb): 2.700
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments:
Insert contains PstI (2), SstI (2), PvuII, and HindIII sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7 doublet, HindIII--4.6, 0.95; BamHI--5.7; PstI--2.85, 1.65, 1.3; XhoI uncut.
The complete insert hybridizes with sequences on X and Y. A PvuII/EcoRI digest yields 2 fragments from the insert--1.7 and 1.0 kb. The larger hybridizes only to the X chromosome. The smaller recognizes all the same fragments as p2A7.
References:

Mohandas TK, et al. Steroid sulfatase gene in XX males. Am. J. Hum. Genet. 46: 369-376, 1990. PubMed: 2301402

Ross MT, et al. Long-range physical mapping around the human steriod sulfatase locus. Genomics 6: 528-539, 1990. PubMed: 2328992

Ballabio A, et al. Isolation and characterization of a steroid sulfatase cDNA clone: genomic deletions in patients with X-chromosome-linked ichthyosis. Proc. Natl. Acad. Sci. USA 84: 4519-4523, 1987. PubMed: 3474618

Fraser N, et al. Identification of incomplete coding sequences for steroid sulphatase on the human Y chromosome: evidence for an ancestral pseudoautosomal gene?. Development 101: 127-137, 1987. PubMed: 3503710