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| 产品名称： | pGBM2 |
|---|---|
| 商品货号： | TS145248 |
| Designations： | pGBM2 |
| Depositors： | J Geiselmann |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 4.0460000038146970 DESCRIPTION OF VECTOR: Intact vector size: 4.046 Type of vector: plasmid Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI Construction: pSC101, pUC18 MCS Host range: Escherichia coli Features (with orientation and position when available): marker(s): strR/spcR, replicon: pSC101 MCS: HindIII...EcoRI Vector: pGBM2 (plasmid) Construction: pSC101, pUC18 MCS Marker(s):spcR,strR Construct size (kb): 4.046000003814697 Features: marker(s): strR/spcR replicon: pSC101 MCS: HindIII...EcoRI |
| Applications： | The vector can be used whenever it is necessary to maintain two plasmids within the same cell. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--2.9, 1.1; AvaII--3.4, 0.6; XbaI--4.0. The vector can be used whenever it is necessary to maintain two plasmids within the same cell. Recommendation for verification: XbaI--4.05; SacI--4.05; BglI--2.94, 1.11; AvaII--3.41, 0.64. ATCC 87497 and 87498 differ in multiple cloning sites. |
| Media： | ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Manen D, et al. A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322. Gene 186: 197-200, 1997. PubMed: 9074496 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |